Master thesis - Design, purification and functional testing

Job description

3374712
Additional Cities

Uppsala
Career Level

Co-op/Intern
Relocation Assistance

No
Business

GE Healthcare
Business Segment

Healthcare BioPharma
Function

Engineering/Technology
Country/Territory

Sweden
Postal Code

751 84
Role Summary/Purpose

Master thesis - Design, purification and functional testing of protein ligands used for purification of glycoproteins

GE Healthcare Life Sciences provides products and services used as tools for biopharmaceutical manufacturing, drug discovery and the latest in cellular technologies, thereby enabling our customers around the world to be more productive, effective and innovative.
Essential Responsibilities

Protein glycosylation is one of the most prevalent posttranslational modification (PTM) and is critical in regulating biological activity. Most extracellular eukaryotic proteins (including antibodies and membrane proteins) are glycosylated and carbohydrate binding ligands are hence attractive for purification application purposes. Several carbohydrate binding proteins (e.g. Lectins) are available for purification of glycoproteins, for example Concanavalin A (ConA). However, ConA-based products suffer from undesired leaching during purification that may contaminate the target glycoprotein eluate. Other carbohydrate binding proteins represent distinct specificities for additional or similar types of sugar moieties and may be suitable as an alternative strategy to ConA-based applications.

The aim of this thesis is to evaluate carbohydrate binding proteins and their suitability in glycoprotein purification.
Methods used will be molecular biology, cultivation of E. coli, expression and purification of proteins and protein analysis.

Master thesis work will be for spring 2020. Deadline for application is 15th November 2019. For questions, please contact Jon Lundqvist: jon.lundqvist@ge.com or Peter Lundbäck: peter.lundbaeck@ge.com.
Qualifications/Requirements

·  The project requires good basic knowledge in molecular biology and chromatography, ability to work independently and good collaboration skills.
About Us

GE (NYSE:GE) drives the world forward by tackling its biggest challenges. By combining world-class engineering with software and analytics, GE helps the world work more efficiently, reliably, and safely. GE people are global, diverse and dedicated, operating with the highest integrity and passion to fulfill GE’s mission and deliver for our customers. www.ge.com

GE Healthcare provides transformational medical technologies and services to meet the demand for increased access, enhanced quality and more affordable healthcare around the world. GE works on things that matter - great people and technologies taking on tough challenges. From medical imaging, software & IT, patient monitoring and diagnostics to drug discovery, biopharmaceutical manufacturing technologies and performance improvement solutions, GE Healthcare helps medical professionals deliver great healthcare to their patients. Learn More About GE Healthcare .

GE is committed to taking on the world’s toughest challenges. In order to fulfill that promise we rely on a culture of leadership, diversity and inclusiveness. We aim to employ the world’s brightest minds to help us create a limitless source of ideas and opportunities. We believe in hiring talented people of varied backgrounds, experiences and styles…people like you!

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Primary Country

Sweden
Primary State/Province

Uppsala
Primary City

Uppsala